"Biotechnology means any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use."
Genetic engineering is an important discipline of biotechnology and has a wide range of applications covering new drug developments, environmental conservation, energy generation and crop improvements yielding plants that could withstand various external insults. Through genetic engineering, a target (recombinant) protein could be produced efficiently using the machinery of the foster host. The engineered host may undergo fermentative cultivation to attain large quantities of the recombinant protein. Subsequent to purification to remove the contaminants, the recombinant protein is attainable as a highly pure, fully bioactive and authentic product.
Ligation of DNA fragments and
introduction into bacterial hosts
A bioreactor for growth
of bacterial cells
A large-scale purification
A chromatographic set-up, which may be employed to separate the recombinant protein from its contaminants